Ok, I injected 6 koi with ovaprim. I probably screwed up on some of them, it takes practice. I did not have the angle on my white red eye kohaku, it look like it slanted skin deep. Ironically it was my 5th try, I was too worried about the scale. Also, I may have pressed the ovaprim out of a few of them. I noticed an air pocket? Next time, I will just press the injection hole as I pull out. Its 8am, I wonder what will happen in 10 hours. I want to play with my hatching jar and try to hand spawn a pair.
As I reflect on the day, I realize my poor performance was a result of rushing. This is the first time working with a chemical base anesthetic, I always use clove oil. The chemical based Trican-S is superior to clove oil. One and a half teaspoon in 5 gallons of water will knock the koi out in less than a minute to the point where the gills stop moving. This freaked me out. I ended up diluting the anesthetic bath with the water from the Japanese koi net. At first I prepared a separate container of fresh water and placed them in there and hand carried them to the bath, so I wouldn't dilute the bath. I found out latter this anesthetic bath can be diluted, it just take a little longer for them to go under. Once they knock out there is no rush, they are out. Its also easier to bring them back with fresh water. I injected them in the water, next time I will take them out, dry them up and take my time.